PCR-based method for isolation of the flagellin genes from Pseudomonas species.

PCR-based assay for the rapid and precise distinction of Pseudomonas aeruginosa from other Pseudomonas species recovered from burns patients

BACKGROUND Pseudomonas aeruginosa is an important lifethreatening nosocomial pathogen which plays a prominent role in wound infections in burns patients. We designed this study to identify the isolates of P. aeruginosa recovered from burns patients at the genus and species levels by means of primers targeting oprI and oprL genes. METHODS During a 5-month period, wound samples were taken from ...

Isolation and Genetic Fingerprinting of Pseudomonas aeruginosa from Iranian Patients with Cystic Fibrosis Using RAPD-PCR

Sixty four Iranian patients with cystic fibrosis (CF) were studied for colonization with Pseudomonasaeruginosa. The patient’s age ranged between 2 months to 18 years old. Twenty one patients werecolonized, 15 with non-mucoid and 6 with mucoid strains of P. aeruginosa. The colonization rateincreased with age and the mucoid phenotype was only recovered from the older patients. A...

Isolation of a new antibiotic from a species of Pseudomonas.

developing a pattern based on speech acts and language functions for developing materials for the course “ the study of islamic texts translation”

هدف پژوهش حاضر ارائه ی الگویی بر اساس کنش گفتار و کارکرد زبان برای تدوین مطالب درس "بررسی آثار ترجمه شده ی اسلامی" می باشد. در الگوی جدید، جهت تدوین مطالب بهتر و جذاب تر، بر خلاف کتاب-های موجود، از مدل های سطوح گفتارِ آستین (1962)، گروه بندی عملکردهای گفتارِ سرل (1976) و کارکرد زبانیِ هالیدی (1978) بهره جسته شده است. برای این منظور، 57 آیه ی شریفه، به صورت تصادفی از بخش-های مختلف قرآن انتخاب گردید...

PCR-based method for isolation of full-length clones and splice variants from cDNA libraries.

Most cDNA library screening procedures do not distinguish between full-length and incomplete clones and therefore may yield incomplete cDNA fragments. Thus, there is a widespread need for a method allowing the efficient selection of full-length clones. I present a rapid, PCR-based method that allows the simultaneous screening of > 10(6) cDNAs. The longest cDNA is identified in the first step so...